FIG. 7.
CBP is the target of competitive recruitment by different transcription factors. (A) RAR signaling is repressed by ligand-activated PPARγ. CV1 cells were cotransfected with 0.2 μg of (DR5)2-luc reporter construct and 1 μg of PPARγ expression plasmid (or empty vector). Cells were treated with 10 μM BRL49653 for 2 h and then induced with 0.1 μM TTNPB for 24 h before harvesting. (B and C) Transactivation of (AOx)3-TK-luc (B) and transrepression of iNOS-luc (C) were differentially affected by overexpression of GAL-CBP and GAL–SRC-1 fusions. (B) RAW 264.7 cells were cotransfected with 0.2 μg of (AOx)3-TK-luc reporter construct, 0.02 μg of PPARγ expression plasmid, and 0.8 μg of GAL fusion construct. Cells were treated with BRL49653 (0.1 μM) for 24 h before harvesting. (C) RAW 264.7 cells were cotransfected with 0.5 μg of iNOS-luc reporter construct, 0.5 μg of PPARγ expression plasmid, and 0.5 μg of GAL fusion construct. Cells were pretreated with BRL49653 (10 μM) for 2 h and then induced with LPS (1 μg/ml) for 24 h before harvesting.