Table 1.
Richness estimators and diversity indices of the 16S rRNA gene libraries in ileal digesta, colonic digesta, and colon mucosa of pigs with different feeding frequenciesa.
| Sample | Items | M1 | M3 | M5 | P-value |
|---|---|---|---|---|---|
| Ileal digesta | Chao 1 | 222.57 ± 34.61 | 202.85 ± 27.29 | 238.03 ± 36.79 | 0.77 |
| Shannon | 2.21 ± 0.21 | 2.43 ± 0.14 | 1.88 ± 0.17 | 0.12 | |
| Colonic digesta | Chao 1 | 1247.18 ± 50.16 | 1296.12 ± 56.61 | 1330.99 ± 66.44 | 0.60 |
| Shannon | 4.94 ± 0.08 | 5.06 ± 0.11 | 5.07 ± 0.12 | 0.64 | |
| Colon mucosa | Chao 1 | 706.21 ± 19.20 | 694.13 ± 25.05 | 664.18 ± 29.81 | 0.49 |
| Shannon | 4.34 ± 0.05 | 4.08 ± 0.09 | 4.06 ± 0.15 | 0.12 |
a
The richness estimators (Chao 1) and diversity indices (Shannon) were calculated. The data were analyzed by one-way ANOVA followed by Duncan's t-tests to evaluate differences in feeding regimens, data are expressed as mean, SEM, n = 8. OTUs, operational taxonomic units; M1, one meal per day; M3, three meals per day; M5, five meals per day.