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Thermodynamic parameters for binding of ubiquitin to REV1 UBM1-UBM2 and REV1 UBM2 obtained by ITC
| ΔGb | ΔHb | −TΔSb | |||
|---|---|---|---|---|---|
| Sample | Site (N)a | KD (μM)b | kcal/mol | ||
| REV1 UBM1-UBM2 (C958S C977A) | 0.8 ± 0.1 | 18.7 ± 1.6 | −6.45 ± 0.05 | −1.10 ± 0.13 | −5.34 ± 0.18 |
| REV1 UBM2 | 0.9 ± 0.1 | 14.9 ± 3.0 | −6.59 ± 0.12 | −1.17 ± 0.64 | −5.41 ± 0.52 |
Binding stoichiometry
KD is the dissociation constant. ΔG, ΔH and ΔS are the respective changes in Gibbs free energy, enthalpy, and entropy upon binding at T=298 K.
