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. 2021 Nov 12;16(11):e0248668. doi: 10.1371/journal.pone.0248668

Fig 3. NLRP3 blockade prevents PKD auto-phosphorylation.

Fig 3

(A) Immunoblot analysis of whole cell lysates from THP-1 cells treated with nigericin for the indicated times ± MCC950 (1 μM), AFN700 (3 μM) or CGP084892 (20 μM), monitoring total PKD3 as well as auto-phosphorylated PKD and gasdermin D cleavage. (B) Immunoblot analysis of whole cell lysates from THP-1-WT or NLRP3-KO cells treated with nigericin for the indicated times, auto-phosphorylated PKD, as well as full and N-terminally cleaved gasdermin D.