FIG. 4.

Effect of PIG compounds on tyrosine phosphorylation of pp125^FAK and paxillin and pp125^FAK-IRS-1 association. Isolated rat adipocytes (A) or nonadherent and adherent 3T3-L1 adipocytes (B) were incubated in the absence or presence of increasing concentrations of PIG compounds. pp125^FAK and paxillin were immunoprecipitated (IP) with corresponding antibodies from defatted cell lysates and immunoblotted (IB) with anti-IRS-1, anti-pp125^FAK (pp125^FAK only), or antiphosphotyrosine antibodies. Phosphorimages of typical experiments are shown (A) repeated two to four times with similar results. Quantitative evaluation is given as fold stimulation (mean ± standard deviation [B]). Basal tyrosine phosphorylation and association are set at 1 in each case.