TABLE 3.

Inhibition of pp59^Lyn by neutralizing antibody in adipocytes^a

Antibody dilution	Phosphorylation (arbitrary units)
	Basal	PIG 41	Insulin
None	8,513 ± 1,112	94,672 ± 12,732	32,462 ± 4,244
Anti-rat IgG (1:25)	8,034 ± 1,034	91,207 ± 13,245	35,785 ± 4,890
Anti-pp59Lyn
1:2,000	8,744 ± 1,055	85,992 ± 11,308	39,833 ± 4,067
1:500	7,231 ± 865	50,315 ± 7,855	23,121 ± 3,133
1:100	6,023 ± 762	37,312 ± 2,988	19,452 ± 2,629
1:50	5,149 ± 441	27,057 ± 3,562	10,038 ± 1,759
1:25	4,540 ± 331	23,560 ± 2,234	8,207 ± 1,552
1:10	4,267 ± 255	20,135 ± 2,433	7,064 ± 970

^aIsolated rat adipocytes were electroporated with neutralizing anti-pp59^Lyn or anti-rat IgG antibody at various dilutions and then incubated (15 min, 37°C) in the absence or presence of PIG 41 (3 μM) or human insulin (10 nM). pp59^Lyn was immunoprecipitated with monoclonal anti-pp59^Lyn antibody from defatted cell lysates and then immunoblotted with antiphosphotyrosine antibody. Autophosphorylated kinase was quantitatively evaluated by phosphorimaging (arbitrary units). The experiment was repeated twice (mean ± standard deviation).