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. 2021 Oct 28;17(10):e1009888. doi: 10.1371/journal.pgen.1009888

Fig 3. Functional analysis of GSTu1 in chlorantraniliprole resistance.

Fig 3

RNAi-mediated knockdown of GSTu1 reduced the resistance of P. xylostella to chlorantraniliprole in NIL (A) and BL(B). C: Expression and purification of GSTu1 in the E. coli system (M: protein marker; lane 1: negative control; lane 2: total soluble proteins; lane 3: purified proteins). D: Enzyme kinetics of GSTu1 with a series of ascending chlorantraniliprole (0–1000 μM) and fixed GSH. E: Depletion rate of chlorantraniliprole by recombinant GSTu1. F: The specific activity of purified GSTu1 toward CDNB. Asterisks indicate significant differences between the treatment and the corresponding control (Student’s t-test, * 0.01 < P < 0.05, **P < 0.01).