Fig 4. Lnc-GSTu1-AS is involved in the regulation of chlorantraniliprole resistance.

Relative expression of lnc-GSTu1-AS at different developmental stages (A), in different tissues (B), in nuclear RNA and cytoplasmic RNA (C), and in control and lnc-GSTu1-AS knockdown groups (D) of P. xylostella. Relative expression of GSTu1 in the control and lnc-GSTu1-AS knockdown groups by qRT-PCR (E) and Western blot (F, I). G: Mortality of P. xylostella treated with the LC₅₀ of chlorantraniliprole. H: Analysis of the binding relationship between lnc-GSTu1-AS and GSTu1-3′ UTR by ribonuclease protection assays (RPA). J: Co-localization of lnc-GSTu1-AS and GSTu1 in the midgut of P. xylostella by fluorescence in situ hybridization (FISH) assay. Asterisks indicate significant differences between the treatment and the corresponding control (Student’s t-test, * 0.01 < P < 0.05, **P < 0.01). Different lowercase letters represent significant differences (one-way ANOVA followed by Tukey’s multiple comparison tests, P < 0.05).