Fig 7. Lnc-GSTu1-AS maintains the stability of GSTu1 through prevention of its degradation by masking the binding site of miR-8525-5p in chlorantraniliprole-resistant P. xylostella.

Relative expression of miR-8525-5p (A) and lnc-GSTu1-AS (B) after inhibition of miR-8525-5p and knockdown of lnc-GSTu1-AS by qRT-PCR. Relative expression of GSTu1 after inhibition of miR-8525-5p combined with knockdown of lnc-GSTu1-AS by qRT-PCR (C) and Western blot assay (D, F). E: Mortality of P. xylostella treated with the LC₅₀ of chlorantraniliprole. Asterisks indicate significant differences between the treatment and the corresponding control (Student’s t-test, ns indicates no significant differences, * 0.01 < P < 0.05, **P < 0.01).