Skip to main content
. 2021 Nov 12;7(46):eabi8602. doi: 10.1126/sciadv.abi8602

Fig. 3. Mitochondrial lactate metabolism supports M2 polarization through ACLY-dependent histone acetylation.

Fig. 3.

(A) Total acetylated histone H3 enzyme-linked immunosorbent assay, (B) lysine residue–specific acetylation immunoblot, and (C) H3K9ac ChIP of BMDMs starved in GF media + 10% dFBS (4 hours) before supplementation with glucose (5 mM) or lactate (10 mM) and polarization with IL-4 (20 ng/ml) for 6 hours. (D) M2 gene expression, (E) lysine residue–specific acetylation, and (F) H3K9ac ChIP of BMDMs starved in GF media + 10% dFBS (4 hours) before pretreatment ± UK-5099 (25 μM), supplementation with lactate (10 mM) or acetate (10 mM), and polarization with IL-4 (20 ng/ml) for 48 (D) or 6 hours (E and F). Data are presented as means ± SEM and represent at least three experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by one-way ANOVA (A and C) or two-way ANOVA (D and F) with Tukey’s post-test.