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. 2021 Nov 12;7(46):eabk0271. doi: 10.1126/sciadv.abk0271

Fig. 9. HSCs are larger in aged humans.

Fig. 9.

(A and B) Mean volume (fl) of human HSCs (Lin−, CD34+, CD90+, CD38−, CD45RA− with and without CD49f+) from young (21 to 25 years, n ≥ 5) or old (51 to 62 years, n ≥ 5) individuals. (C) Colony-forming efficiency in vitro: Human HSCs from young (21 to 25 years) or old (51 to 62 years) individuals were plated onto methylcellulose, and the percentage of HSCs forming a colony was quantified after 21 days (n = 3). (D) Differentiation assay in vitro: Percentages of human GEMM, GM, or single lineages (G, M, and E) colonies per plate were counted after 21 days (n = 3). CFU, colony-forming units; BFU, burst-forming unit; GEMM, = granulocyte, erythroid, macrophage, megakaryocyte; G, granulocytes; M, macrophages. E, erythroid.