Figure 2.

Pterostilbene attenuates inflammatory response in the LPS stimulated 16HBE cells. The 16HBE cells were treated with LPS with or without Pts (15 or 30 μM). (A) Cell viability was detected by MTT and represented by relative absorbance to control. (B) Determination of nitrite in 16HBE cells by the Griess reagent. (C) PGE2 production by 16HBE cells was measured with ELISA. (D) The iNOS and COX‐2 expressions were detected by Western blot. (E) Contents of IL‐6, TNF‐α, and IL‐1β was detected by ELISA. (F) Cell cycle of 16HBE cells was measured by flow cytometry. All data were expressed as mean ± SD. *p < .05, compared with the Control group. ^# p < .05, compared with the LPS group. ELISA, enzyme‐linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide; Pts, Pterostilbene; TNF, tumor necrosis factor