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. 2021 Oct 6;478(19):3555–3573. doi: 10.1042/BCJ20210375

Figure 2. GZD-824 suppresses LRRK2 mediated Rab10 phosphorylation without inhibiting Ser935 phosphorylation.

Figure 2.

(A,B) Wild-type MEFs were treated with or without the indicated concentrations of inhibitors for 2 h. Cells were lysed, and 20 µg of extract was subjected to quantitative immunoblot analysis with the indicated antibodies (all at 1 µg/ml). Each lane represents cell extract obtained from a different dish of cells (three replicates per condition). The membranes were developed using the Odyssey CLx Western Blot imaging system. (C) As in (A) except that cells were treated with 1 μM GZD-824 or 100 nM MLi-2 for the indicated times. (D) As in (A) except that cells were treated with 1 μM GZD-824 or 100 nM MLi-2 or 100 nM GSK3357679A for 24 h. (A–D) Immunoblots were quantified using the Image Studio software. Data are presented relative to the phosphorylation ratio observed in cells treated with DMSO (no inhibitor), as mean ± SD. (A–C) SD are derived from the replicates show in in the presented blots. (D) SD are derived from duplicates run in independent gels.