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. 2021 Oct 6;478(19):3555–3573. doi: 10.1042/BCJ20210375

Figure 3. GZD-824 does not inhibit Ser935 phosphorylation in neutrophils.

Figure 3.

(A) Primary neutrophils from two healthy donors were isolated as described previously [54], and treated with or without 1 μM GZD-824 or 300 nM MLi-2 for 45 min at ambient temperature for the indicated times. Cells were lysed and 25 µg of extract was subjected to quantitative immunoblot analysis with the indicated antibodies (all at 1 µg/ml). The membranes were developed using the Odyssey CLx Western Blot imaging system. Immunoblots were quantified using the Image Studio software. Data are presented relative to the phosphorylation ratio observed in cells treated with DMSO (no inhibitor).