| Antibody |
Anti-Cardiac Troponin T (Rabbit polyclonal) |
Abcam |
ab45932 |
FC (1:200) |
| Antibody |
Anti-Sarcomeric Alpha Actinin (Mouse monoclonal) |
Sigma-Aldrich |
A7811 |
IF (1:200) |
| Antibody |
Cleaved Caspase-3 (Asp175) (Rabbit polyclonal) |
Cell Signaling Technology |
9661 |
FC (1:800) |
| Antibody |
GAPDH (Mouse monoclonal) |
Santa Cruz Biotechnology |
sc-47724 |
WB (1:1000) |
| Antibody |
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control |
Abcam |
ab170190 |
FC (1:2000) |
| Antibody |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Rabbit polyclonal) |
Cell Signaling Technology |
9101 |
WB (1:1000) |
| Antibody |
p44/42 MAPK (Erk1/2) (Total Erk) (Rabbit polyclonal) |
Cell Signaling Technology |
9102 |
WB (1:1000) |
| Antibody |
Phospho-Histone H3 (Ser10) (6G3) (Mouse monoclonal) |
Cell Signaling Technology |
9706 |
FC (1:1000) |
| Antibody |
Phospho-mTOR (Ser2448) (Rabbit polyclonal) |
Cell Signaling Technology |
2971 |
WB (1:1000) |
| Antibody |
Phospho-S6 Ribosomal Protein (Ser235/236) (Rabbit polyclonal) |
Cell Signaling Technology |
2211 |
WB (1:1000)IF (1:100) |
| Antibody |
Rabbit IgG, polyclonal - Isotype Control |
Abcam |
ab37415 |
FC (1:2000) |
| Antibody |
Recombinant Anti-active YAP1 [EPR19812] (Rabbit polyclonal) |
Abcam |
ab205270 |
WB (1:1000)IF (1:500) |
| Antibody |
Recombinant Anti-AKT1 (phospho S473) [EP2109Y] (Rabbit monoclonal) |
Abcam |
ab81283 |
WB (1:5000) |
| Antibody |
Recombinant Anti-AKT1+ AKT2+ AKT3 [EPR16798] (Rabbit monoclonal) |
Abcam |
ab179463 |
WB (1:10,000)IF (1:100) |
| Antibody |
Recombinant Anti-Cyclin D2 (Rabbit monoclonal) |
Abcam |
ab207604 |
WB (1:1000)IF (1:100) |
| Antibody |
Recombinant Anti-Vimentin [EPR3776] (Rabbit monoclonal) |
Abcam |
ab92547 |
IF (1:500) |
| Antibody |
YAP (D8H1X) (Total Yap) (Rabbit monoclonal) |
Cell Signaling Technology |
14074 |
WB (1:1000)IF (1:200) |
| Antibody |
β-Catenin (Carboxy-terminal Antigen) (Rabbit polyclonal) |
Cell Signaling Technology |
9587 |
WB (1:1000) |
| Chemical compound, drug |
SCH772984 |
Cayman Chemical |
19166 |
|
| Chemical compound, drug |
PD0325901 |
Sigma-Aldrich |
PZ0162-5MG |
|
| Commercial assay, kit |
Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit |
Thermo Fisher Scientific |
C10419 |
|
| Commercial assay, kit |
Click-iT EdU Alexa Fluor 488 Imaging Kit |
Thermo Fisher Scientific |
C10337 |
|
| Cell line (Homo sapiens, male) |
DU11 iPSC Line |
Duke University Stem Cell Core |
|
|
| Cell line (H. sapiens) |
Hek293T |
ATCC |
CRL-3216 |
|
| Strain, strain background (Rattus Norvegicus) |
P2 Sprague-Dawley Rat Pups |
Charles River |
|
|
| Recombinant DNA reagent |
pLV-beta-catenin deltaN90 |
pLV-beta-catenin deltaN90 was a gift from Bob Weinberg |
Addgene plasmid # 36985 |
Used to generate new plasmids |
| Recombinant DNA reagent |
HER2 CA (V659E) |
HER2 CA (V659E) was a gift from Mien-Chie Hung |
Addgene plasmid # 16259 |
Used to generate new plasmids |
| Recombinant DNA reagent |
R777-E020 Hs.CCND2-nostop |
R777-E020 Hs.CCND2-nostop was a gift from Dominic Esposito |
Addgene plasmid # 70304 |
Used to generate new plasmids |
| Recombinant DNA reagent |
pSV2 neuNT |
pSV2 neuNT was a gift from Bob Weinberg |
Addgene plasmid # 10,918 |
Used to generate new plasmids |
| Recombinant DNA reagent |
pCMV-flag YAP2 5SA |
pCMV-flag YAP2 5SA was a gift from Kunliang Guan |
Addgene plasmid # 27371 |
Used to generate new plasmids |
| Recombinant DNA reagent |
Pax2 |
psPAX2 was a gift from Didier Trono |
Addgene plasmid # 12260 |
Used to generate lentivirus in HEK293Ts |
| Recombinant DNA reagent |
VSVG |
pMD2.G was a gift from Didier Trono |
Addgene plasmid # 12259 |
Used to generate lentivirus in HEK293Ts |
| Recombinant DNA reagent |
Control Lentivirus (MHCK7-mCherry) |
This manuscript |
|
See Materials and methods section |
| Recombinant DNA reagent |
Cyclin Lentivirus (MHCK7-Ccnd2-P2A-mCherry-NLS) |
This manuscript |
|
See Materials and methods section |
| Recombinant DNA reagent |
B-catenin Lentivirus (MHCK7- Ctnnb1(∆90)-P2A-mCherry-NLS) |
This manuscript |
|
See Materials and methods section |
| Recombinant DNA reagent |
Human Erbb2 Lentivirus (MHCK7-Erbb2(V659E-P2A-mCherry)) |
This manuscript |
|
See Materials and methods section |
| Recombinant DNA reagent |
Rat Erbb2 Lentivirus (MHCK7-Erbb2(V663E-P2A-mCherry-NLS)) |
This manuscript |
|
See Materials and methods section |
| Recombinant DNA reagent |
Yap Lentivirus (MHCK7-Yap8SA-P2A-mCherry-NLS) |
This manuscript |
|
See Materials and methods section |
| Software, algorithm |
CellProfiler |
McQuin et al., 2018
|
https://cellprofiler.org/
|
|
| Software, algorithm |
ImageJ |
Schneider et al., 2012
|
https://imagej.nih.gov/ij/
|
|
| Software, algorithm |
FlowJo Software |
The FlowJo team, 2021
|
|
|
| Software, algorithm |
GraphPad Prism |
|
https://www.graphpad.com/
|
|
| Software, algorithm |
Matlab |
|
|
|
| Sequence-based reagent |
Yap1 |
|
PrimerBank ID: 303523510c1 |
F:TAGCCCTGCGTAGCCAGTTAR:TCATGCTTAGTCCACTGTCTGT |
| Sequence-based reagent |
Myh6 |
|
NCBI PrimerBlast |
F:GCCCTTTGACATTCGCACTGR:GGTTTCAGCAATGACCTTGCC |
| Sequence-based reagent |
Myh7 |
|
PrimerBank ID: 115496168c1 |
F:ACTGCCGAGACCGAGTATGR:GCGATCCTTGAGGTTGTAGAGC |
| Sequence-based reagent |
Ctgf |
|
PrimerBank ID: 98986335c1 |
F:CAGCATGGACGTTCGTCTGR:AACCACGGTTTGGTCCTTGG |
| Sequence-based reagent |
Cyr61 |
|
PrimerBank ID: 197313774c3 |
F:CAGCATGGACGTTCGTCTGR:AACCACGGTTTGGTCCTTGG |
| Sequence-based reagent |
Ctnnb1 |
|
PrimerBank ID: 148233337c2 |
F:CATCTACACAGTTTGATGCTGCTR:GCAGTTTTGTCAGTTCAGGGA |
| Sequence-based reagent |
Lef1 |
|
PrimerBank ID: 260656055c1 |
F:AGAACACCCCGATGACGGAR:GGCATCATTATGTACCCGGAAT |
| Sequence-based reagent |
Axin2 |
|
PrimerBank ID: 195927058c1 |
F:CAACACCAGGCGGAACGAAR:GCCCAATAAGGAGTGTAAGGACT |
| Sequence-based reagent |
Ccnd2 |
|
PrimerBank ID: 209969683c3 |
F:TTTGCCATGTACCCACCGTCR:AGGGCATCACAAGTGAGCG |
| Sequence-based reagent |
Erbb2 |
|
PrimerBank ID: 54792097c2 |
F:TGTGACTGCCTGTCCCTACAAR:CCAGACCATAGCACACTCGG |
| Sequence-based reagent |
Epha2 |
|
PrimerBank ID: 296010835c2 |
F:AGAGGCTGAGCGTATCTTCATR:GGTCCGACTCGGCATAGTAGA |
| Sequence-based reagent |
Epha4 |
|
PrimerBank ID: 45439363c3 |
F:GCAAGGAGACGTTTAACCTGTR:CTTGGGTGAAGCTCTCATCAG |
| Sequence-based reagent |
Fn1 |
|
PrimerBank ID: 47132556c2 |
F:AGGAAGCCGAGGTTTTAACTGR:AGGACGCTCATAAGTGTCACC |
| Sequence-based reagent |
Dusp5 |
|
PrimerBank ID: 62865889c2 |
F:GCCAGCTTATGACCAGGGTGR:GTCCGTCGGGAGACATTCAG |
| Sequence-based reagent |
Dusp6 |
|
PrimerBank ID: 42764682c1 |
F:GAAATGGCGATCAGCAAGACGR:CGACGACTCGTATAGCTCCTG |
| Sequence-based reagent |
Spry4 |
|
PrimerBank ID: 188595696c1 |
F:TCTGACCAACGGCTCTTAGACR:GTGCCATAGTTGACCAGAGTC |
| Sequence-based reagent |
Etv5 |
|
PrimerBank ID: 194018465c1 |
F:TCAGCAAGTCCCTTTTATGGTCR:GCTCTTCAGAATCGTGAGCCA |
| Sequence-based reagent |
Phlda1 |
|
PrimerBank ID: 83977458c1 |
F:GAAGATGGCCCATTCAAAAGCGR:GAGGAGGCTAACACGCAGG |
| Sequence-based reagent |
Spry2 |
|
PrimerBank ID: 22209007c1 |
F:CCTACTGTCGTCCCAAGACCTR:GGGGCTCGTGCAGAAGAAT |
| Sequence-based reagent |
Dusp4 |
|
PrimerBank ID: 325651887c1 |
F:GGCGGCTATGAGAGGTTTTCCR:TGGTCGTGTAGTGGGGTCC |
| Sequence-based reagent |
Etv4 |
|
PrimerBank ID: 118918427c2 |
F:CAGTGCCTTTACTCCAGTGCCR:CTCAGGAAATTCCGTTGCTCT |
| Sequence-based reagent |
Fos |
|
PrimerBank ID: 254750707c2 |
F:GGGGCAAGGTGGAACAGTTATR:CCGCTTGGAGTGTATCAGTCA |
| Sequence-based reagent |
Dab2 |
|
PrimerBank ID: 349585059c1 |
F:GTAGAAACAAGTGCAACCAATGGR:GCCTTTGAACCTTGCTAAGAGA |
| Sequence-based reagent |
Pdgfa |
|
PrimerBank ID: 197333758c1 |
F:GCAAGACCAGGACGGTCATTTR:GGCACTTGACACTGCTCGT |
| Sequence-based reagent |
Runx1 |
|
PrimerBank ID: 169790826c1 |
F:CTGCCCATCGCTTTCAAGGTR:GCCGAGTAGTTTTCATCATTGCC |
| Sequence-based reagent |
Hprt1 |
|
PrimerBank ID: 164518913c1 |
F:CCTGGCGTCGTGATTAGTGR:AGACGTTCAGTCCTGTCCATAA |
