Figure 6. Gene set enrichment analysis (GSEA) reveals long-term sepsis-induced differences in molecular pathways of pre-existing memory CD8 T cells.
Top 5 KEGG pathways positively- (A) and negatively- (D) enriched in CLP hosts. Enrichment scores for Ribosomal- (B) and Adhesion- (E) associated genes. Red box indicates leading edge of enriched region; genes enriched in CLP - box to right, genes enriched in Sham – box to left. Gene expression heatmap of core enriched genes for Ribosomal (C) and Adhesion (F) associated genes.
Figure 6—source data 1. Source data for Figure 6.
elife-70989-fig6-data1.xlsx (21.1KB, xlsx)
Figure 6—figure supplement 1. Gene set enrichment analysis (GSEA) reinforces that sepsis promotes a shift to TCM at transcriptional level.
The KAECH_DAY15_EFF_VS_MEMORY_CD8_TCELL was used for evaluation. KAECH_DAY15_EFF_VS_MEMORY_CD8_TCELL _UP represents genes enriched in effector CD8 T cells relative to memory CD8 T cells. KAECH_DAY15_EFF_VS_MEMORY_CD8_TCELL _DN represents genes enriched in memory CD8 T cells relative to efector CD8 T cells. Enrichment scores for KAECH_DAY15_EFF_VS_MEMORY_CD8_TCELL _UP- (A) and KAECH_DAY15_EFF_VS_MEMORY_CD8_TCELL _DN- (C) associated genes. Red box indicates leading edge of enriched region; genes enriched in CLP - box to right, genes enriched in Sham – box to left. Gene expression heatmap of core enriched genes for Ribosomal (B) and Adhesion (D) associated genes.
Figure 6—figure supplement 1—source data 1. Source data for Figure 6—figure supplement 1A-D.
elife-70989-fig6-figsupp1-data1.xlsx (29.4KB, xlsx)