Figure 5.

Expression of senescence-related markers in Tsc1-cKO retina. (a, b) Western blot analysis showed the upregulation of p21 and p16Ink4a protein in 5-month-old Tsc1-cKO and 24-month-old C57BL/6 (old) retina compared to age-matched control (Ctrl). The experiment was repeated twice with two different samples in each group. ^∗p < 0.05 by unpaired Students' t-test between groups. (c) SA-β-gal staining of the 5-month-old Tsc1-cKO retina and age-matched control. Strong positive signals were found in GCL and INL (arrows in (c)) of the Tsc1-cKO retina. No distinctive signal was found in photoreceptor cells. Scale bar: 100 μm.