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TMEM206 knockdown attenuates the Wnt/β-catenin signaling pathway. Wnt/β-catenin signaling pathway protein level was dramatically reduced in TMEM206-shRNA-transfected U2OS and MG63 cells (∗∗∗P < 0.001, as comparing with SC-shRNA group, respectively). GAPDH was used as an internal control. The data are represented as means ± SD in (b–f).
