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. 2021 Nov 5;2021:9311855. doi: 10.1155/2021/9311855

Figure 2.

Figure 2

Berberine reduced iNOS expression and TNF-α release and upregulated SOCS1 expression in Aβ-treated microglial cells. The N9 microglia were divided into five groups, including the normal cultured control group, 5 μM Aβ group, and three concentrations of berberine treatment groups (medium containing 0.1 μM, 1 μM, or 5 μM berberine plus 5 μM Aβ); after 24 h treatment, iNOS and SOCS1 expressions were measured by using Western blot, and TNF-α release was assessed by ELISA. Then, the cells were divided into four groups, including the control and 0.1 μM, 1 μM, and 5 μM berberine treatment groups; after 24 h incubation, the MTT assay was performed to evaluate the cell injury level. (a) Microglial iNOS protein expression (n = 4). (b) TNF-α concentration in the medium (n = 8). (c) SOCS1 protein expression (n = 4). (d) Cell viability assay (n = 8). Results are expressed as means ± SD. ∗P < 0.05; NS: no significance.