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. 2021 Nov 5;2021:9311855. doi: 10.1155/2021/9311855

Figure 4.

Figure 4

SOCS1-siRNA reversed BBR-induced effects on iNOS expression and proinflammatory factor releases. The cells were divided into five groups, including the normal cultured control group, 5 μM Aβ exposure group, BBR treatment group (medium containing 5 μM BBR and 5 μM Aβ), SOCS1 treatment group (microglial cells were treated with SOCS1-siRNA for 6 h and then exposed to the medium containing 5 μM BBR and 5 μM Aβ), and scrambled- (SC-) siRNA treatment group (microglial cells were treated with SC-siRNA for 6 h and then exposed to the medium containing 5 μM BBR and 5 μM Aβ); then, after 24 h treatment, iNOS expression was observed by using immunocytochemistry staining and Western blot, and inflammatory factors were assessed by using ELISA. (a) Immunocytochemistry staining of microglial iNOS expression. (b) Western blot result of iNOS expression (n = 4). (c–e) TNF-α, IL-1β, and IL-6 concentrations in the medium (n = 8). Results are expressed as means ± SD. Bar = 10 μm; ∗P < 0.05; NS: no significance.