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. 2021 Nov 5;2021:9311855. doi: 10.1155/2021/9311855

Figure 5.

Figure 5

SOCS1-siRNA reversed BBR-induced effects on Arg-1 expression and neurotrophic factor releases. The cells were divided into five groups, including the normal cultured control group, 5 μM Aβ treatment group, BBR treatment group (medium containing 5 μM BBR and 5 μM Aβ), SOCS1 treatment group (microglial cells were treated with SOCS1-siRNA for 6 h and then exposed to the medium containing 5 μM BBR and 5 μM Aβ), and scrambled- (SC-) siRNA treatment group (microglial cells were treated with SC-siRNA for 6 h and then exposed to the medium containing 5 μM BBR and 5 μM Aβ); then, after the treatments, Arg-1 expression was measured by using immunocytochemistry staining and Western blot, and neurotrophic factors were assessed by using ELISA. (a) Immunocytochemistry staining of microglial Arg-1 expression. (b) Western blot result of Arg-1 expression (n = 4). (c–e) BDNF, GDNF, and IL-10 concentrations in the medium (n = 8). Results are expressed as means ± SD. Bar = 10 μm; ∗P < 0.05; NS: no significance.