Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 12;11:22154. doi: 10.1038/s41598-021-01689-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Transfection conditions can strongly influence prime editing efficiency in hiPSC. (A) Analysis of mK02 gene correction in two temporally separated experiments of hiPSC-TLR transfected with pCMV-PE2 prime editor and g+4 R20P13_dP pegRNA. (B) Tolerance of prime editing efficiency to pipetting inaccuracy in hiPSC-TLR. Cells were transfected with different volumes of Lipofectamine Stem (LFS) and different amounts of DNA per well of 12-well. (C, D) Dependency of transfection efficiency and prime editing efficiency to number of cells seeded the day before transfection. Data are represented as ± SD from n = 3 (A, C, D) or n = 6 (3B) and significance was calculated using 1-way ANOVA with Tukey's post-test or 2-way ANOVA with Bonferroni multiple comparisons test for data displayed as groups (****p ≤ 0.0001; *p ≤ 3.05; ns: p > 0.05).