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. 2021 Nov 12;12:6564. doi: 10.1038/s41467-021-26883-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Geographical (coloured circle) and ecological (coloured name) origins of the parental strains (left) used for generating the diploid backgrounds (right). Diploids are grouped according to their level of heterozygosity as: homozygous parents (light brown), intraspecies hybrids (brown) and interspecies hybrids (dark brown) and the same colour codes apply to b, d and e. b Level of heterozygosity across the hybrid panel with the number of markers detected in non-collinear and collinear regions. Each data point is labelled with a number/colour encoded according to a. The four homozygous parents are not reported. c URA3-loss assay used for measuring RTG-induced recombination rates. d Top: the y axis reports in logarithmic scale the percentage of cells growing in 5-FOA measured with the URA3-loss assay and quantified with the LOH difference (R_T6−R_T0) where R_T6 and R_T0 are the LOH rates at T6 and T0, respectively (Supplementary notes). Error bars represent standard deviations. Each point represents the average of n = 5 independent replicates. Samples having a negative LOH difference (non-significant differences between control and RTG sample) are not reported. Middle: cell growth quantified with the natural logarithm of the LOH ratio (R_T6/R_T0). Error bars represent standard deviations. Each point represents the average of n = 5 independent replicates. The number on the top of each bar indicates the linear fold increase. Bottom: meiotic progression after 12 h measured as the percentage of cells that passed the first (MI) and the second (MII) meiotic divisions (quantified with fluorescence microscopy of DAPI-stained cells). e Correlation between the meiotic progression (MI + MII cells after 12 h in sporulation medium) and the LOH difference (top, logarithmic scale) as well as the natural logarithm of the LOH ratio (bottom). r is the Pearson’s correlation coefficient, the p value of the correlation is calculated as a two-sided test. In both plots, the grey area represents the 95% confidence interval. Samples showing negative LOH differences were removed to avoid a bias towards correlation.