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. 2021 Nov 12;11:22195. doi: 10.1038/s41598-021-01690-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of Spike-pseudotyped VSV(S) penetration into human A549 lung cell by cardiac glycosides. (a) Measurement of inhibition by luminescence background subtract. VSV(S) was pre-treated with drugs for 1 h, and then exposed to cells in DMEM for 2 h. VSV(S) mixture was then replaced by whole medium and incubated for 24 h. Data are the averages of N = 3 independent experiments. Error bars are ± SE. (b) Measurement by luminescence ratio. (c) Measurement by % of positive control. (d) Negative control with mouse 3T3 cells missing hACE2, measured by luminescence background subtract method.