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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Methods. 2021 May 13;196:104–112. doi: 10.1016/j.ymeth.2021.05.009

Figure 1:

Figure 1:

(A) Trans RNA ligation of two RNAs to form a circular RNA. If two transcripts can be trans ligated at both ends, then a circular RNA is formed that contains both sequences. (B) Split Broccoli is only fluorescent when both Broccoli A and Broccoli B are present. Both Broccoli A and Broccoli B are essential to form the DFHBI-binding site (two G-quartets and the U-A-U triplet). A stable and fluorescent RNA is only formed after trans ligating Broccoli A and Broccoli B into a circular RNA.