FIG. 4.

JNK activation by Msn(Δ332–667) in 293 cells. Msn(wt) or various Msn mutant constructs (0.1 μg each) were transfected into 293 cells together with 10 ng of a plasmid expressing a fusion protein of ATF2 and the GAL4 DNA binding domain and 5 μg of a plasmid expressing a GAL4 luciferase reporter. Transfection efficiency was assessed by coexpressing 1 μg of a plasmid expressing β-galactosidase. The means from two experiments performed in triplicate are shown. Luciferase activity is expressed in arbitrary units after being standardized to β-galactosidase activity.