Table 1.

sgRNA sequences used for gene editing. Oligonucleotide sequences used in RT-PCR, RT-qPCR and gDNA amplification.

Name	Sequence 5′ → 3′	Sense
sgRNA sequences
1A- sgRNA	CACACGGAAACTTAGGGCTA	Forward
1B- sgRNA	GGAGATGTCCTGGACTCACA	Forward
2A- sgRNA	TGGGGAGAATGAGTCCGGGT	Reverse
2B- sgRNA	GGACTTTAGAACCAGGACCG	Reverse
Oligonucleotide sequences
257 F	GGAAGAGGAGACACGGAACA	Forward
Ex 1-F	CCTGGACTCACACGGAAACT	Forward
Ex 2-F	GGACTCATTCTCCCCAGACG	Forward
526 R	CCTTTGTTCAGCCACATTGG	Reverse
257 R	TGTTCCGTGTCTCCTCTTCC	Reverse
CRD1 F	TGAGGAAAAGGAGCAGAGGA	Forward
CRD1 R	AGAGACCAGTTTGCTTTTTGTT	Reverse
CRD2 F	GGAGCTTGTTGCCAGAGAGT	Forward
CRD2 R	CACTGGAGGGT GTGAGAACC	Reverse
CRR1 F	TTTCCGATCACGAGACTAGC	Forward