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. 2021 Nov 12;4:1280. doi: 10.1038/s42003-021-02810-x

Fig. 5. Integrated ligand-receptor analysis in sc/snRNAseq data compendium informs colocalized communication analysis in spatial transcriptomics data.

Fig. 5

a Predicted ensemble ligand-receptor interactions strengths between endothelial, FAP, myeloid, smooth muscle (ligand-expressing), and myogenic (receptor-expressing) cell types using CellChat. b Chord plot showing the significant pathways annotated as “Secreted Signaling” between FAP and myogenic sub-populations, as assessed by CellChat (see “Methods”). Chords point from the FAP ligand source (protein name abbreviations listed; MK, midkine) to the myogenic cell sub-population expressing its partner receptor (names not listed). The width of the chords shows the interaction strength. c Relative interaction strengths of specific Midkine ligand-receptor pairs summed across all FAP and myogenic populations. d Log-normalized expression of the receptor and e ligand gene transcripts are shown for the sc/snRNAseq data compendium, for the four myogenic cell types, and the three FAP cell types, respectively. f Log-normalized expression of Midkine signaling factors in spatial RNAseq data (at 2, 5, and 7 dpi).