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Chemicals used for amplification of foraminiferal COI fragments; concentrations and volumes are shown per sample.
| Chemicals | End concentration | Volume |
|---|---|---|
| MQ water | 11.7 μl | |
| PCR buffer CL | 10× | 2.0 μl |
| MgCl2 | 25 mM | 0.4 μl |
| BSA | 10 mg/ml | 0.8 μl |
| Forward primer | 10 pMol/μl | 1 μl |
| Reverse primer | 10 pMol/μl | 1 μl |
| dNTPs | 2.5 mM | 0.4 μl |
| Qiagen Taq | 5 U/μl | 0.2 μl |
| DNA template | 5 μl |
