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. 2021 Nov 12;11:22171. doi: 10.1038/s41598-021-00126-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal dynamic of UVA1 dose effects on NAD(P)H and FAD metabolic biomarkers in basal keratinocytes of reconstructed human skin. Representative images of (a–c) NAD(P)H and FAD fluorescence intensities and (d–f) fB fractions of bound NAD(P)H and FAD acquired before (T₀), at 30 min (T_expo + 30 min) and 2 h (T_expo + 2 h) post sham-exposure (Control) or UVA1 exposure at 25 J/cm² and 40 J/cm². (g–i) Representative phasor plot scatters of NAD(P)H and (j–l) FAD fluorescence lifetime data of the same samples at T₀, T_expo + 30 min and T_expo + 2 h. Every pixel of the phasor plot is color coded according to the fraction of bound metabolites with the same look up table as for images (d)–(f).