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. 2021 Nov 12;11:22171. doi: 10.1038/s41598-021-00126-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of a long UVA filter on NAD(P)H and FAD metabolic stress response to UVA1. (a–c) Example of NAD(P)H and FAD fluorescence intensities and (d–f) fractions of bound NAD(P)H and FAD before (T₀) and 2 h (T_expo + 2 h) post sham-exposure (Control), UVA1 40 J/cm² exposure in the presence and absence of a long UVA sun filter. (g–i) Quantification of fractions of bound NAD(P)H and FAD of the same samples at three experimental time points (T₀, T_expo + 30 min and T_expo + 2 h). Data are presented as boxplots. (j–l) Histological HES (hematoxylin eosin saffron) analysis of the same reconstructed human skin samples imaged by FLIM, replaced in culture and fixed at 2 days post exposure.