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. 2021 Nov 12;7:351. doi: 10.1038/s41420-021-00733-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The protein abundance of DACH1 was examined by western blot in T47D, ZR-75-30, MCF-7 cell lines. b, c Scratch wound-healing assay assessing the effects of DACH1 overexpression or knockdown on the motility of ZR-75-30 and MCF-7 cells. d Transwell migration and invasion assays evaluating the effects of DACH1 on the motility and invasion of ZR-75-30 cells. Cell migration and invasion assays were performed in 24-well chambers without or with Matrigel. Cells were transfected with Flag-DACH1 or control vector and then inoculated into the upper chamber. After cultured 24 h, cells on the lower surface of the filter were stained and photographed. The scale bars stand for 100 μm. Experiments were performed in triplicates and the data are representative of three independent experiments.