Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 12;7:351. doi: 10.1038/s41420-021-00733-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a ZR-75-30 cells were transfected with Flag-DACH1 or control vector, and then subjected to the analysis of MMP9 and MMP2 expression by RT-PCR. b Western blot assay evaluating the protein level of MMP9 in ZR-75-30 cells transfected with Flag-DACH1 or control vector (the first three lines). The medium from this assay was subjected to gelatin zymography (the last line). c Breast cancer cells were transfected with MMP-Luc together with or without Flag-DACH1 (left) or sh-DACH1 (right). Luciferase activity was measured 24 h after transfection. d HEK293T cells were transfected with MMP-Luc along with 0, 200, 400, 600 ng Flag-DACH1, and the luciferase activity was measured 24 h after transfection. There were three independent experiments with three parallel wells each. The data are representative of three independent experiments. Data are presented as means ± SD (P < 0.05, significant; ns, not significant).