Figure 2.

Multiple T cell subsets express Hopx. Cells from thymi, spleens, and peripheral lymph nodes of Hopx^GFPFoxp3^RFP mice were analyzed by flow cytometry. (A) (Left) Representative plots show Hopx (GFP) and Foxp3 (RFP) expression among all T cells (gated as single, live, CD3ε⁺, NK1.1^neg) from thymus (top), spleen (middle), or lymph nodes (bottom). Shaded regions indicate population further characterized on the right (Hopx⁺Fopx3⁺ - blue and Hopx⁺Foxp3^neg – red). (Right) Representative plots show anti-CD4 and anti-CD8α staining intensity among Hopx⁺Fopx3⁺ and Hopx⁺Foxp3^neg T cells from the thymus, spleen, and lymph nodes. (B) Graphs show the percentages of CD4⁺ cells among Hopx⁺Fopx3⁺ (left) and Hopx⁺Foxp3^neg (right) T cells from the thymus, spleen, or lymph nodes as indicated (n = 5–6 mice from three independent experiments). (C) Graph shows the percentages of CD8α⁺ cells among Hopx⁺Foxp3^neg T cells from the thymus, spleen, or lymph nodes as indicated (n = 5–6 mice from three independent experiments). (D) Representative overlaid histograms show Hopx (GFP) expression among CD8α⁺ (gray) and CD4⁺Foxp3^neg (red) T cells from the thymus, spleen, or lymph nodes as indicated. (E) Graphs show the percentages of Hopx⁺ cells among CD4⁺Foxp3^neg (left) and CD8α⁺ (right) T cells from the thymus, spleen, or lymph nodes as indicated (n = 5–6 mice from three independent experiments). (B, C, and E) Graphs show mean ± SD. ns – not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 determined by one-way ANOVA with Tukey's multiple comparisons.