Figure 5.

Variegated Hopx expression pattern in myeloid cells. Cells from spleens of Hopx^GFPFoxp3^RFP mice were analyzed by flow cytometry. (A) Gating strategy for identification of myeloid cell populations. Representative plots (pre-gated on single, live, CD3ε^neg, NK1.1^neg, CD19^neg, B220^neg, CD45⁺ cells) show staining intensity of the indicated fluorochrome-conjugated antibodies for the indicated populations. Shaded regions indicate corresponding populations analyzed in B and C. (B) Representative overlaid histograms show Hopx (GFP) expression among the indicated cell types. (C) Graph shows the percentages of Hopx⁺ cells among the indicated cell populations (n = 5 mice from two independent experiments). Graph shows mean ± SD. ∗∗P < 0.01 and ∗∗∗∗P < 0.0001 determined by one-way ANOVA with Tukey's multiple comparisons.