KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD11c, clone N418 | Biolegend | Cat# 117336, RRID: AB_2565268 |
| Anti-mouse I-Ab, clone AF6-120.1 | Biolegend | Cat# 116422, RRID: AB_10613473 |
| Anti-mouse CD64, clone X54-5/7.1 | Biolegend | Cat# 139323, RRID: AB_2629778 |
| Anti-mouse F4/80, clone BM8 | Biolegend | Cat# 123118, RRID: AB_893477 |
| Anti-mouse Ly-6G/Ly-6C (Gr-1); clone RB6-8C5 | Biolegend | Cat# 108441, RRID: AB_2562401 |
| Anti-mouse CD8α, clone 53-6.7 | Biolegend | Cat# 100733, RRID: AB_2075239 |
| Anti-Mouse CD45R/B220, clone RA3-6B2 | Biolegend | Cat# 103208, RRID: AB_312993 |
| Anti-Mouse CD45R/B220, clone RA3-6B2 | Biolegend | Cat# 103243, RRID:AB_11203907 |
| Anti-mouse CD19, clone 6D5 | Biolegend | Cat# 115508, RRID:AB_313643 |
| Anti-mouse CD19, clone 6D5 | Biolegend | Cat# 115555, RRID:AB_2565970 |
| Anti-mouse CD4, clone GK1.5 | Biolegend | Cat# 100447, RRID:AB_2564586 |
| Anti-mouse CD3ε, clone 145-2C11 | Biolegend | Cat# 100308, RRID:AB_312673 |
| Anti-mouse CD3ε, clone 145-2C11 | BD Biosciences | Cat# 564378, RRID:AB_2738779 |
| Anti-mouse NK1.1, clone PK136 | Biolegend | Cat# 108707, RRID: AB_313394 |
| Anti-mouse NK1.1, clone PK136 | Biolegend | Cat# 108709, RRID:AB_313396 |
| Anti-mouse IgD, clone 11.26C.2A | Biolegend | Cat# 405719, RRID:AB_2561875 |
| Anti-mouse CD24, clone M1/69 | Biolegend | Cat# 101823, RRID:AB_1595596 |
| Anti-mouse KLRG1, clone 2F1/KLRG1 | Biolegend | Cat# 138415, RRID:AB_2561735 |
| Anti-mouse CD16/32, clone 2.4G2 | N/A | Produced in-house from hybridomas obtained from ATCC |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Critical commercial assays | ||
| Zombie Aqua™ Fixable Viability Kit | BioLegend | Cat#: 423102 |
| Deposited data | ||
| scRNA-seq of whole CD45+ splenocytes from B6 mice, 10X (HMS) | ImmGen Consortium (Heng and Painter, 2008) | |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Mouse: Foxp3IRES-mRFP | Jackson Laboratory | Stock# 008374; (Wan and Flavell, 2005) |
| Mouse: Hopx3FlagGFP | available at Jackson Laboratory on a mixed background | Stock# 029271; (Takeda et al., 2013) |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| FlowJo 10 | FlowJo, LLC | https://www.flowjo.com; |
| GraphPad Prism | GraphPad Software | https://www.graphpad.com; |
| Seurat v3 | (Stuart et al., 2019) | https://satijalab.org/seurat/ |
| R v4.0.2 | The R Foundation | https://www.r-project.org |
| Other | ||
| Roche Collagenase D | MilliporeSigma | Cat#: 11088882001 |