Fig. 2. BH3-mimetics and BH3-only proteins can upregulate anti-apoptotic BCL-2 proteins in a non-cell autonomous manner.

a Schematic of supernatant transfer experiments: HeLa tBID2A cells were treated with 500 nM venetoclax for 3 h, followed by exchange to regular growth medium for 45 h. Supernatant was harvested, filtered and added onto recipient cells before analysis. b HeLa tBID2A cells were treated directly with venetoclax or with supernatant from untreated or venetoclax treated control or BAX BAK^CRISPR cells as described in a. After 48 h cells were harvested and protein expression was analysed by western blot (representative blot of three independent repeats). c Supernatant from control or venetoclax treated cells was digested with Proteinase K before addition onto recipient cells and protein expression was analysed by western blot after 48 h (representative blot of three independent repeats). d Supernatant from HeLa cells transfected with tBID-GFP was collected after 48 h and transferred onto recipient HeLa cells. After 48 h, recipient cells were harvested and protein expression analysed by western blot. Fold change normalised to loading control is stated below (representative blot of two independent repeats).