Fig. 5. Non-cell autonomous upregulation of BCL-2 proteins is independent of transcription.

a HeLa tBID2A cells were treated with 500 nM venetoclax for the indicated time, RNA was harvested and expression of FGF2 mRNA analysed by qPCR. n = 4 independent experiments; mean values ± s.e.m.; *p < 0.0001; unpaired, two-sided t-test. b HeLa tBID2A cells were treated with 500 nM venetoclax for the indicated time, harvested and protein expression analysed by western blot. Fold change normalised to loading control is stated below (representative blot of three independent repeats). c HeLa tBID2A cells were treated with venetoclax ± actinomycin D for 3 h, followed by 45 h incubation in regular medium. Then the supernatant was harvested, filtered and added onto recipient cells. After 48 h, recipient cells were harvested and protein expression analysed by western blot (representative blot of three independent repeats). d HeLa tBID2A cells were treated with venetoclax for 3 h, followed by 45 h incubation in regular medium. Then the supernatant was harvested, filtered, supplemented with actinomycin D as indicated and added onto recipient cells. After 3 h, recipient cells were harvested and protein expression analysed by western blot (representative blot of three independent repeats).