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. 2021 Nov 12;12:6556. doi: 10.1038/s41467-021-26908-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Pseudo-colour plot of NP-specific GC B cells in the spleen of HuR^fl/fl and HuR^fl/fl AID^Cre mice at day 10 after immunisation with NP-KLH in alum. Right panels show the percentage of GC B cells based on surface expression or not of IgG1 and binding of NP antigen. Data are representative from 1 of the 3 independent experiments performed, n = 9 mice per group and experiment, two-sided Mann–Whitney test). b Histograms showing HuR expression in splenic FO and GC B cells from HuR^fl/fl and HuR^fl/fl AID^Cre mice at day 7 and day 14 after immunisation. Right panel, the percentage of HuR⁺ and HuR⁻ GC B cells in HuR^fl/fl and HuR^fl/fl AID^Cre mice at day 14 after immunisation (data representative from 1 of the 3 independent experiments performed (n = 9 mice per group and experiment)). c Pseudo-colour plots and analysis of the proportion of CD45.2⁻ and CD45.2⁺ GC B cells in draining LNs of BM chimera mice (WT CD45.1⁺/HuR^fl/fl CD45.2⁺ or CD45.1⁺/HuR^fl/fl AID^Cre CD45.2⁺, ratio 1:3) after immunisation with NP-KLH in alum. d Percentage of WT (CD45.2⁻) GC B cells and HuR^fl/fl or HuR^fl/fl AID^Cre (CD45.2⁺) GC B cells expressing the cell surface markers of DZ and LZ GC B cells in BM chimera mice. Data are presented as mean values +/− SEM. e Analysis of the proportion of CD45.2⁻ and CD45.2⁺ NP-specific IgG1⁺ GC B cells in mice shown in c. Data in c–e are from n = 12 or 16 mice per group depending on the genotype and the day of analysis. Data in e are from n = 12 mice per group. Two-sided Mann–Whitney test in d. f Myc⁺ GC B cells in draining LNs of HuR^fl/fl Myc^GFP/GFP and HuR^fl/fl Myc^GFP/GFP AID^Cre mice at day 7 after immunisation with NPKLH in alum (s.c.). Top panels, representative contour plots of Myc staining and GFP detection (previously gated on CD19⁺ IgD⁻ CD95⁺ CD38⁻ cells). Bottom panels, percentage and number of Myc⁺ GC B cells (n = 7 (HuR^fl/fl) or n = 8 (HuR^fl/fl AID^Cre) mice, two-sided Mann–Whitney test). Source data are provided as a Source data file.