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. 2021 Nov 12;12:6556. doi: 10.1038/s41467-021-26908-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Cumulative distribution of fold change in the expression of Myc-dependent genes (Hallmark gene set) in LZ and DZ GC B cells (Kolmogorov–Smirnov test). b Cumulative distribution of changes in the expression of Myc-dependent genes that are targets or not of HuR (Kolmogorov–Smirnov test). c Enrichment analysis of genes related to cell cycle (GSEA, Gene set from Reactome). d Quantitation from our transcriptome analysis of the changes in the expression of HuR mRNA targets associated with the cell cycle phase G1–S in HuR KO DZ GC B cells compared to control cells (only HuR mRNA targets identified in all conditions of B cells treated with LPS, αCD40+IL4 or αIgM+IL4 were considered). e BrdU incorporation in GC B cells from HuR^fl/fl and HuR^fl/fl AID^Cre mice at day 7 after immunisation with NP-KLH in alum. Left panels, representative histograms of BrdU staining in total GC B cells or in DZ and LZ GC B cells. Right panel, percentage of BrdU⁺ cells in the cell populations indicated (data from 2 independent experiments, n = 11 (HuR^fl/fl) or n = 9 (HuR^fl/fl AID^Cre) mice, two-sided Mann–Whitney test). f Strategy for labelling proliferating GC B cells in vivo with EdU and BrdU. g Analysis by flow cytometry of cycling GC B cells from HuR^fl/fl and HuR^fl/fl AID^Cre mice at day 7 after immunisation. Left panels, representative contour plots of BrdU and EdU incorporation by cycling GC B cells. Right panels, percentage of cells labelled with EdU, BrdU and EdU/BrdU (data from 2 independent experiments, n = 16 (HuR^fl/fl) or n = 13 (HuR^fl/fl AID^Cre) mice, two-sided Mann-Whitney test). Source data are provided as a Source data file.