REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-AGO2	Abcam	Cat# ab186733; RRID:AB_2713978
anti-BCL2L2	Proteintech	Cat# 16026-1-AP; RRID: AB_2827646
anti-CDK4	Proteintech	Cat# 11026-1-AP; RRID:AB_2078702
anti-NFkappaB p65 (RELA)	Santa Cruz	Cat# sc-8008; RRID:AB_628017
anti- Histone H3	Santa Cruz	Cat# sc-10809; RRID:AB_2115276
anti-CDK6	Santa Cruz	Cat# sc-177; RRID:AB_631225
anti-HNRNPA2B1	Santa Cruz	Cat# sc-53531; RRID:AB_2248245
anti-DHX9	Santa Cruz	Cat# sc-137232; RRID:AB_2261698
anti-ACTB	Santa Cruz	Cat# sc-47778; RRID:AB_626632
anti-VHL	Santa Cruz	Cat# sc-135657; AB_2215955
anti-AURKA	Santa Cruz	Cat# sc-56881; RRID:AB_667838
anti-RELA(p65)	Santa Cruz	Cat# sc-8008; RRID:AB_628017
anti-HNRNPL	Santa Cruz	Cat# sc-32317; RRID:AB_627736
anti-Flag	Santa Cruz	Cat# sc-807; RRID:AB_675756
Anti-rabbit IgG, HRP-linked Antibody	Santa Cruz	Cat# sc-2004; RRID:AB_631746
Anti-mouse IgG, HRP-linked Antibody	Santa Cruz	Cat# sc-2005; RRID:AB_631736
Chemicals, Peptides, and Recombinant Proteins
Antibiotic (Penicillin/Streptomycin)	Thermo Fisher	Cat# 15140122
NP40 Thermo Scientific	Thermo Fisher	Cat# 85124
Lipofectamine2000	Thermo Fisher	Cat# 11668019
PBS	Thermo Fisher	C10010500BT
Trypsin	Thermo Fisher	Cat# 15400054
DMEM	Thermo Fisher	Cat# C11995500BT
PRIM1640	Thermo Fisher	Cat# C11875500BT
RNase inhibitors	Thermo Fisher	Cat# EO0381
Bis-TrisNuPage 4-12% gel	Thermo Fisher	Cat# NP0335BOX
TRIzol reagent	Thermo Fisher	Cat# 15596026
GlycoBlue coprecipitant	Thermo Fisher	Cat# AM9516
SuperScript III reverse transcriptase	Thermo Fisher	Cat# 18080044
Pre-staining protein marker 10 to 180kDa	Thermo Fisher	Cat# 26617
20X SSC BUFFER	Thermo Fisher	Cat# AM9763
HEPES (1 M)	Thermo Fisher	Cat# 15630080
TRIS-HCL PH 7.5 1M	Thermo Fisher	Cat# 15567027
1 M MAGNESIUM CHLORIDE	Thermo Fisher	Cat# AM9530G
2 M KCL	Thermo Fisher	Cat# AM9640G
10X TBE	Thermo Fisher	Cat# AM9865
RNASE T1	Thermo Fisher	Cat# EN0542
RNase A/T1	Thermo Fisher	Cat# EN0551
EDTA	Thermo Fisher	Cat# AM9260
RNase-free DNase I	Thermo Fisher	Cat# 18068015
streptavidin Dynabeads beads C1	Thermo Fisher	Cat# 65002
Proteinase K Invitrogen	Thermo Fisher	Cat# 25530049
Flag antibody coated M2 beads	Sigma Aldrich	Cat# M8823
paraformaldehyde (PFA)	Sigma Aldrich	Cat# 158127
Triton X-100	Sigma Aldrich	Cat# T8787
TWEEN 20	Sigma-Aldrich	Cat# P9416
DMSO	Sigma Aldrich	Cat# D2650
NaCl	Sigma Aldrich	Cat# S5150-1L
KCl	Sigma Aldrich	Cat# P9333
Protease inhibitor cocktail	Sigma Aldrich	Cat# P8340
20xSSC Buffer Sigma Aldrich	Sigma Aldrich	Cat# 93017
glycine	Sigma Aldrich	Cat# G8898
Glycerol	Sigma Aldrich	Cat# G5516-500ML
Tris-EDTA 0.5M pH 8.0	Sigma Aldrich	Cat# 93283-500ML
2-Mercaptoethanol	Sigma Aldrich	Cat#M6250
Biotin RNA Labeling Mix	Roche	Cat# 11685597910
Yeast tRNA	Roche	Cat# 10109495001
Fetal Bovine Serum	PAN Biotech	Cat# ST30-3302
Moloney Murine Leukemia Virus Reverse Transcriptase	Promega	Cat# M1705
PVDF membrane	Millipore	Cat# ISEQ00010
Propidium Iodide	Beyotime	Cat# ST511
Critical Commercial Assays
Cell Light EdU DNA imaging Kit	RiboBio	Cat# C10310-3
Cell Counting Kit-8 (CCK-8) assay kit	Dojindo	Cat# CK04
ECL western blotting Kit (, Thermo Scientific Pierce)	Thermo Fisher	Cat# 32106
SYBR Green Supermix	Promega	Cat# S2062,
Dual-Glo luciferase assay kit	Promega	Cat# E1980
T7 transcript KIT	TOYOBO	Cat# TSK-101B
KOD plus neo KIT	TOYOBO	Cat# KOD-401S
Biological samples
Patient-derived samples	The First Affiliated Hospital of Guangzhou Medical University (Ren et al. 2014)	N/A
Experimental Models: Cell Lines
NCI-HCC827	ATCC	Cat# CRL-2868
A549	ATCC	Cat# CCL-185
NCI-H661	ATCC	Cat# HTB-183
NCI-H1299	ATCC	Cat# CRL-5803
NCI-H358	ATCC	Cat# CRL-5807
NCI-H1975	ATCC	Cat# CRL-5908
NCI-H23	ATCC	Cat# CRL-5800
HCC827	ATCC	Cat# CRL-2868
MRC-5	ATCC	Cat# CCL-171
HLF	ATCC	Cat# CCL-153
HeLa	ATCC	Cat# CCL-2
HEK-293	ATCC	Cat# CRL-1573
95D	This paper	N/A
PC9	This paper	N/A
Oligonucleotides
No target control (NC)	RiboBio	Cat# siN0000001-1-5
hsa-miR-506-3p mimic	RiboBio	Cat# miR10002878-1-5
hsa-miR-34a-5p mimic	RiboBio	Cat# miR10000255-1-5
hsa-miR-195-5p mimic	RiboBio	Cat# miR10000461-1-5
si-h-CDK6_001	RiboBio	Cat# siB084281913
si-h-CDK6_002	RiboBio	Cat# siB084281932
si-h-CDK6_003	RiboBio	Cat# siB084281948
si-h-HNRNPA2B1_001	RiboBio	Cat# siB160409043647
si-h-HNRNPA2B1_002	RiboBio	Cat# siB160409043801
si-h-HNRNPA2B1_003	RiboBio	Cat# siB160706015613
si-h-DHX9_001	RiboBio	Cat# stB0006120A
si-h-DHX9_002	RiboBio	Cat# stB0006120B
si-h-DHX9_003	RiboBio	Cat# stB0006120B
CDK6 FISH Cy3 probes, see Table S3	Generay biotechnology	N/A
Primers for RT-qPCR, see Table S4	This paper	N/A
Recombinant DNA
pENTER-HNRNPA2B1-Flag	Vigene Biosciences	Cat# CH865056
pENTER-GFP-Flag	This paper	N/A
pET28a-GFP	This paper	N/A
pET28a-HNRNPA2B1	This paper	N/A
Software and Algorithms
ImageJ	ImageJ	https://imagej.nih.gov/ij/
Bio-RAD CFX Manager	BIO-RAD	http://www.bio-rad.com/en-us/product/cfx-manager-software?tab=Download
ZEN	Zeiss	https://www.zeiss.com/microscopy/int/software-cameras.html
Accuri C6 Plus	BD Biosciences	http://www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd accuri/m/1294932/overview
MACS2 (v2.1.0)	GitHub	https://github.com/taoliu/MACS/
ChIPSeeker (v1.14.2)	Bioconductor	http://www.bioconductor.org/packages/release/bioc/html/ChIPseeker.html
FlowJo Software	FlowJo	https://www.flowjo.com/