FIGURE 1.

Reduced NAMPT expression and NAD⁺ content in oocytes from HFD mice. (a) Western blotting showing the NAMPT expression in ND and HFD oocytes. GAPDH served as a loading control. Band intensity was calculated using Image J software, the ratio of NAMPT/GAPDH expression was normalized, and values are indicated (200 oocytes per lane). (b) Confocal sections of ND and HFD oocytes stained with NAMPT antibody (green) and counterstained with propidium iodide (red) for DNA. Arrowheads indicate the accumulated NAMPT signal. Scale bars: 30 µm. (c) Quantification of NAMPT immunofluorescence (n = 25 oocytes for ND and HFD). (d) NAD⁺ content in ND and HFD oocytes. Data are expressed as the mean ± SD from three independent experiments. In c, statistical analysis was performed with two‐way ANOVA; and in d, a Student's t test (two‐tailed) was used for statistical analysis. **p < 0.01, ***p < 0.001. HFD, high‐fat diet; NAMPT, nicotinamide phosphoribosyl transferase; ND, normal diet