FIG. 6.

Bcl-2 directly enhances p27 levels, independently of p130-mediated cell cycle effects. The p130^−/− fibroblast-transfected lines were either growing asynchronously (asyn) or had been stimulated for the indicated times with 10% FBS to reenter the cell cycle. The immunoblots (50 μg of protein per lane) involved a 4 to 20% polyacrylamide gradient minigel (Novex). The line separating the 16-h and 20-h lanes indicates that the data came from different gels run simultaneously in the same tank. The less pronounced effect of Bcl-2 at the 16-h time point compared to later times was not seen in other experiments and reflects experimental variation.