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. 2021 Nov 11;218(12):e20210920. doi: 10.1084/jem.20210920

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© 2021 Ma et al.

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Figure 2. — Peripheral nerve injury–caused ZNF382 down-regulation contributes to neuropathic pain. (A–C) Effect of premicroinjection with AAV5-Znf382 (Znf382) or control AAV5-Gfp (Gfp) into the ipsilateral L4 DRG on the development of SNL-induced mechanical allodynia (A and B) and heat hyperalgesia (C) at different days before or after SNL or sham surgery on the ipsilateral side of male mice. n = 12 mice/group. Data from two independent experiments. Two-way repeated-measure (RM) ANOVA followed by post hoc Tukey test. ***, P < 0.001 versus the SNL plus Gfp group at the corresponding time points. (D–F) Effect of premicroinjection with AAV5-Znf382 (Znf382) or AAV5-Gfp (Gfp) into the ipsilateral L4 DRG on the development of SNL-induced mechanical allodynia (D and E) and heat hyperalgesia (F) on the ipsilateral side of female mice. n = 12 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by post hoc Tukey test. **, P < 0.01; ***, P < 0.001 versus SNL plus Gfp group. (G–I) DRG microinjection with AAV5-Znf382 (Znf382) mitigated the maintenance of SNL-induced mechanical allodynia (G and H) and heat hyperalgesia (I). n = 12 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by post hoc Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus SNL plus Gfp group. (J–L) Effect of microinjection with AAV5-Znf382 shRNA (shRNA) or control AAV5-scrambled shRNA (Scr) into the unilateral L3/L4 DRGs of naive male mice on the paw withdrawal responses to mechanical (J and K) and heat (L) stimuli. n = 12 mice/group. Data from three independent experiments. Two-way RM ANOVA followed by post hoc Bonferroni’s test. **, P < 0.01; ***, P < 0.001 versus the Scr group. (M–O) Effect of microinjection with AAV5-Znf382 shRNA (shRNA) or AAV5-scrambled shRNA (Scr) into the ipsilateral L3/L4 DRGs of naive female mice on the paw withdrawal responses to mechanical (M and N) and heat (O) stimulation on the ipsilateral side. n = 12 mice/group. Data from two independent experiments. Two-way RM ANOVA followed by post hoc Bonferroni’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus the Scr group.