Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 11;218(12):e20210920. doi: 10.1084/jem.20210920

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Ma et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — ZNF382 determines the HDAC1-mediated ac-H3 enrichment in the Cxcl13 promoter and 5′-UTR. (A) Fragments immunoprecipitated by anti-ac-H3 antibody from the Cxcl13 promoter and 5′-UTR in mice L4 DRGs. n = 2 repeats (12 mice). Data are representative of two independent experiments. Input: total purified fragments. M, ladder marker. (B) The increased level of ac-H3 in F11 and F3 in the ipsilateral L4 DRG on day 7 after SNL. n = 3–5 repeats (18–30 mice)/group. Data from three independent experiments. *, P < 0.05; ***, P < 0.001. Two-tailed unpaired Student’s t test. (C) The decreased abundance of HDAC1 in F11, but not F3, in the ipsilateral L4 DRG on day 7 after SNL. n = 3 repeats (18 mice)/group. Data from two independent experiments. *, P < 0.05. Two-tailed unpaired Student’s t test. (D and E) Effect of AAV5-Znf382 (Z) or AAV5-Gfp (Gfp) on the abundances of HDAC1 (D) and ac-H3 (E) in F11 in the ipsilateral L4 DRG on day 7 after SNL or sham surgery. n = 3 repeats (18 mice)/group. Data from two independent experiments. *, P < 0.05; **, P < 0.01. Two-tailed unpaired Student’s t test. (F and G) Effect of AAV5-Znf382 shRNA (shRNA) or AAV5-scrambled shRNA (Scr) on the abundances of HDAC1 (F) and ac-H3 (G) in F11 in the ipsilateral L3/L4 DRGs 8 wk after microinjection. n = 3 repeats (9 mice)/group. Data from two independent experiments. *, P < 0.05; **, P < 0.01. Two-tailed unpaired Student’s t test. (H) Effect of Hdac1 siRNA (siHD1) or scrambled siRNA (Scr) on the abundance of ac-H3 in F11 on day 5 after SNL surgery in the ipsilateral L4 DRG premicroinjected with AAV5-Znf382 (Z). n = 3 repeats (18 mice)/group. Data from two independent experiments. **, P < 0.01. Two-tailed unpaired Student’s t test.