Figure 7.

Neither the PDZ nor the FAB domains are required for Cno localization/function during cellularization. Stage 5. (A, D, G, and J) Cross sections. (B, E, H, and K) En face. SAJ. (B’, E’, H’, and K’) 33.33% below SAJs. (C, F, I, and L) MIP. (M–R) Line traces and heatmap quantification of Arm (M–O) or Cno (P–R) mean intensity along lateral membranes. Each column and each line trace is an embryo (n = 7). (A–C) WT. Cno and Arm are enriched apically (A–C, yellow arrows). Arm is also at basal junctions (red arrows). Cno localizes to bicellular and TCJs at the level of SAJs (B) and is enriched at TCJs deeper into embryos (B’ and C). (D–L) CnoWT (D–F), CnoΔPDZ (G–I), and CnoΔFAB (J–L) localize like WT Cno. All also have normal Arm enrichment in apical SAJs and basal junctions.