Figure 8.

Deleting the PDZ has only modest effects on AJ integrity. (A and B) Stage 7. cnoΔPDZ. No defects in mesoderm invagination. (C and D) Stage 8. cnoΔPDZ. Epithelial integrity was largely normal. (E) Stage 7. (F) Stage 8. Occasional gaps or broadening of TCJs (arrows). Near-normal Baz planar polarity (F’″ and F″″). (G and H) Stage 9. cnoΔPDZ. Subtle increase in embryos with multiple cells simultaneously rounded up to divide (H, arrows; 42% vs. 30% in WT). (I and J) Stage 10. cnoΔPDZ appeared normal. (K–N) Quantification of Gaps (K), Baz and Arm planar polarity (L and M), or cell elongation (N). (K–N) Brown Forsythe and Welch ANOVA statistical test, and n indicates the number of fields/embryos analyzed. (N) cnoWT and cnoΔRA were not significantly different from WT. cnoΔPDZ and cnoΔFAB were not significantly different from cnoWT. (K and N) Error bar represent SD. (L and M) The box shows the 25th–75th percentile, the whiskers are 5th–95th percentiles, the horizontal line is the median, and the plus sign (+) is the mean.