Table 4. Antibodies and probes used in this study.
| Antibodies/probes | Species | Dilution | Source |
|---|---|---|---|
| Anti-Cno | Rabbit | 1:1,000 (IF, WB) | Sawyer et al., 2009 |
| Anti-Bazooka | Rabbit | 1:2,000 (IF) | Choi et al., 2013 |
| Anti-Armadillo (N2 7A1) | Mouse2a | 1:50 (IF), 1:500 (WB) | Developmental Studies Hybridoma Bank |
| Anti-Pyd (PYD2) | Mouse2b | 1:250 (IF), 1:500 (WB) | Developmental Studies Hybridoma Bank |
| Anti – α-tubulin (T6199) | Mouse | 1:5,000 (WB) | MilliporeSigma |
| Anti-GFP (JL-8) | Mouse2a | 1:1,000 (WB) | Clontech Laboratories |
| Anti-GFP (4C9) | MouseG1 | 1:50 (IF) | Developmental Studies Hybridoma Bank |
| Anti-GFP (Ab13970) | Chicken | 1:1,000 (IF) | Abcam |
| Secondary antibodies | |||
| Alexa Fluor 488, 568, and 647 | 1:1,000 (IF) | Life Technologies | |
| IRDye 680DR | Rabbit | 1:15,000 (WB) | LI-COR Biosciences |
| IRDye 800CW | Mouse | 1:15,000 (WB) | LI-COR Biosciences |
| Cy3 | Mouse | 1:200 (IF, eyes) | Jackson ImmunoResearch Laboratories |
IF, immunofluorescence; WB, Western blot.