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. 2021 Nov 13;14:154. doi: 10.1186/s13048-021-00917-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Circ_0000745 directly binds to miR-3187-3p in OC cells. A, sub-cellular localization of circ_0000745 in OC cells examined by a nuclear-cytoplasmic RNA separation assay; B, intersections of the candidate target miRNAs of circ_0000745 predicted using StarBase and CircBank; C, correlations between the candidate miRNAs and the survival rate of OC patients predicted on the Pan-cancer platform on StarBase; D, miR-3187-3p expression in the collected tissue samples detected by RT-qPCR; E, a negative correlation between the expression of miR-3187-3p and circ_0000745 in OC tissues; F, expression of miR-3187-3p in ES-2 and SK-OV-3 cells after oe-circ or sh-circ transfection determined by RT-qPCR; G, transfection efficacy of miR-mimic in 293 T cells confirmed by RT-qPCR; H, putative binding site between miR-3187-3p and circ_0000745 and the MT sequence for luciferase assay; I, luciferase activity of circ-WT and circ-MT in 293 T cells examined by the luciferase reporter gene assay. Data were exhibited as mean ± SD from three repetitions. Differences were analyzed by paired t test (D), unpaired t test (G), one-way ANOVA (F) or two-way ANOVA (I); *p < 0.05 vs. adjacent; @ p < 0.05 vs. NC mimic; #p < 0.05 vs. oe-NC; &p < 0.05 vs. sh-NC; In panel E, the correlation between miR-3187-3p and circ_0000745 was validated using Pearson’s correlation analysis, r = − 0.653, p < 0.001