Table 1.
Primer sequences for RT-qPCR
| Gene | Primer sequence (5′-3′) |
|---|---|
| circ_0000745 | F: ATGTTGAAAGTAGCCCGAGCAG |
| R: TGGGAGTGTTGGAAGAAGTTGG | |
| SPECC1 | F: ACCCCAGGAAATCAGTGTCCA |
| R: GTTCCCGAACTTGGGACTCAA | |
| miR-3187-3p | F: CCTGGGCAGCGTGTGGCT |
| R: CTCAACTGGTGTCGTGGA | |
| ERBB4 | F: GGAGTATGTCCACGAGCACAAG |
| R: CGAGTCGTCTTTCTTCCAGGTAC | |
| IGF2BP2 | F: GTTGGTGCCATCATCGGAAAGG |
| R: TGGATGGTGACAGGCTTCTCTG | |
| GAPDH | F: GGGAAACTGTGGCGTGAT |
| R: GAGTGGGTGTCGCTGTTGA | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT |
Note: RT-qPCR Reverse transcription quantitative polymerase chain reaction, SPECC1 Sperm antigen with calponin homology and coiled-coil domains 1, miR microRNA, ERBB4 Erb-b2 receptor tyrosine kinase 4, IGF2BP2, insulin like growth factor 2 mRNA binding protein 2, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, F Forward, R Reverse